Detection of UTF1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line untreated (panel A) or treated with 10mM retinoic acid for 3 days (panel B) was stained with Sheep Anti-Mouse UTF1 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.  UTF1 expression is decreased with retinoic acid treatment, as indicated in panel B. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

UTF1 in D3 Mouse Stem Cells. UTF1 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse UTF1 Antigen Affinity-purified Polyclonal Antibody at 10ug/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody. Cells were double-stained using Mouse Anti-Human/Mouse SSEA-1 Monoclonal Antibody and the Northern­Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody. Specific staining of UTF1 was localized to nuclei.